T4 RNA Ligase Antibodies

Target Information

T4 RNA ligase is an enzyme derived from bacteriophage T4, which is primarily involved in the catalysis of RNA molecules. This ligase facilitates the joining of single-stranded RNA or DNA by forming phosphodiester bonds between 3'-hydroxyl and 5'-phosphate termini. Structurally, T4 RNA ligase is composed of distinct domains that contribute to its function as a ligase, including a catalytic domain essential for the reaction. It is frequently employed in molecular biology laboratories for constructing cDNA libraries, RNA sequencing, and cloning applications due to its ability to ligate oligonucleotides, ribonucleotides, and adaptors efficiently. T4 RNA ligase has also been instrumental in site-specific labeling and the study of RNA structure and function, including splicing mechanisms and RNA repair processes. Its versatility as a biotechnological tool is underscored by its high efficiency and specificity, making it a critical component in RNA research and applications. Advances in understanding the enzyme's mechanism have facilitated the development of improved ligation methods, enhancing various RNA technologies.